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Sondes Section 2
YSI Incorporated Environmental Monitoring Systems Operations Manual 2-48
RHODAMINE WT 2-POINT
Select Rhodamine from the Calibrate Menu and then 2-2-Point.
NOTE: One standard must be 0 ug/L in rhodamine WT, and this standard must be calibrated first.
To begin the calibration, place the correct amount (see Tables 1-8) of 0 standard (clear deionized or
distilled water) into the calibration cup provided with your sonde and immerse the sonde in the water.
Input the value 0 ug/L at the prompt, and press Enter. The screen will display real-time readings that will
allow you to determine when the readings have stabilized. Activate the wiper 1-2 times by pressing 3-
Clean Optics as shown on the screen, to remove any bubbles. After stabilization is complete, press Enter
to “confirm” the first calibration and then, as instructed, press Enter to continue.
Dry the sonde carefully and then place the sonde in the second rhodamine WT standard (100 ug/L is
recommended) using the same container as for the 0 ug/L standard. Input the correct rhodamine WT
concentration in ug/L, press Enter, and view the stabilization of the values on the screen in real-time. As
above, activate the wiper with the “3” key or manually rotate the sonde to remove bubbles. After the
readings have stabilized, press Enter to confirm the calibration and then press Enter to return to the
Calibrate menu.
Thoroughly rinse and dry the calibration cups for future use.
- 6-Series 1
- User Manual 1
- SAFETY NOTES 2
- TABLE OF CONTENTS 3
- SECTION 1 INTRODUCTION 5
- 1.1 ABOUT YSI INCORPORATED 5
- 1.2 HOW TO USE THIS MANUAL 5
- Introduction Section 1 6
- SECTION 2 SONDES 7
- 2.1 GETTING STARTED 7
- 2.2 CONNECTING YOUR SONDE 8
- You will need 9
- Sonde to 650 Display/Logger 10
- PROTECTIVE CAP 11
- DRY MEMBRANE 11
- TRANSPORT CUP 14
- BULKHEAD WITH 14
- PROBE PORT PLUGS 14
- ISE PORT PLUG 15
- Figure 20B 17
- Figure 20C 17
- LUBRICATE O-RINGS 20
- OPTIC 21
- BULKHEAD 22
- PROBE GUARD 22
- 2.3.3 STEP 3 - POWER 23
- 24
- SONDE BODY 27
- BATTERY CAP 27
- BULKHEAD CONNECTOR 27
- WITH CAP 27
- 2.5 SONDE SOFTWARE SETUP 31
- SONDE MENU FLOW CHART 34
- 2.6.1 INTRODUCTION 40
- USING THE CALIBRATION CUP 41
- Sampling Applications 47
- TURBIDITY 2-POINT 51
- RHODAMINE WT 2-POINT 54
- 2.7 TAKING READINGS 55
- ANALYZE DATA 60
- OPENING A DATA FILE 63
- VIEWING DATA 64
- 2.9 SONDE MENU 74
- 2.9.1 RUN 75
- UNATTENDED SAMPLING 77
- 2.9.2 CALIBRATE 81
- ROX OPTICAL DISSOLVED OXYGEN 83
- PRESSURE – ABS AND GAGE 87
- AMMONIUM 88
- NITRATE 89
- CHLORIDE 90
- OPTIC TURBIDITY 90
- OPTIC CHLOROPHYLL 91
- OPTIC BGA-PC 92
- OPTIC BGA-PE 93
- 2.9.3 FILE 98
- 2.9.4 STATUS 101
- 2.9.5 SYSTEM 102
- Sondes Section 2 103
- 2.9.7 SENSOR 106
- O-RING CARE AND MAINTENANCE 118
- SONDE PROBE PORTS 119
- CABLE CONNECTOR PORT 119
- 6562 RAPID PULSE DO PROBES 119
- DEPTH SENSOR 124
- LEVEL SENSOR 124
- PLIERS (SLIP-JAW) 125
- AMMONIUM OR 125
- NITRATE PROBE 125
- 2.10.3 SONDE STORAGE 126
- 2.10.4 PROBE STORAGE 127
- 3.1 INTRODUCTION 131
- 3.2 GETTING STARTED 131
- 3.2.1 UNPACKING 132
- 3.2.3 650 CONFIGURATIONS 133
- 650 MDS Section 3 134
- 3.2.4 650 FEATURES 136
- (4) Screws 138
- 3.3 SETTING UP THE 650 148
- 3.4 SONDE MENU INTERFACE 151
- 3.4.2 SONDE MENU EXAMPLES 153
- SELECTION 155
- 3.5.2.1 INTRODUCTION 159
- MEMORY 160
- 3.5.3.1 INTRODUCTION 163
- 3.6 MANAGING 650 FILES 176
- 3.6.2 DIRECTORY 177
- 3.6.3 UPLOAD TO PC 179
- 3.6.4 VIEW FILE 180
- 3.6.5 FILE MEMORY 181
- 3.6.6 DELETE ALL FILES 181
- 3.7.1 INTRODUCTION 182
- 3.7.2 UPLOAD PROCEDURE 182
- 3.8 USING GPS WITH THE 650 183
- 3.11 TROUBLESHOOTING 188
- SAVE THESE INSTRUCTIONS! 190
- Restrictions on Usage 190
- 4.1.2 GLOSSARY 197
- 4.1.3 TUTORIAL 198
- 4.1.4 USING THE TOOLBAR 199
- 4.1.5 TYPICAL APPLICATION 201
- 650 MDS to Lab Computer 203
- 4.2.4 USING THE GRAPH 205
- 4.3 ECOWATCH MENU 208
- COPY TO CLIPBOARD 210
- SAVE DATA DISPLAY 211
- LOAD DATA DISPLAY 211
- PRINT SETUP 215
- 4.3.2 EDIT 217
- 4.3.3 VIEW 218
- STUDY INFO 220
- 4.3.4 COMM(UNICATION) 221
- 4.3.5 REAL-TIME 227
- 4.3.6 GRAPH 228
- 4.3.7 SETUP 229
- 4.3.8 APPL(ICATIONS) 236
- 4.3.9 WINDOW 237
- 4.3.10 HELP 238
- 5.1 CONDUCTIVITY 239
- 5.2 SALINITY 240
- 5.5 pH 242
- 5.6 DEPTH AND LEVEL 244
- 5.7 TEMPERATURE 245
- POLAROGRAPHIC 245
- Winkler titration 246
- FLOW DEPENDENCE 247
- 5.10 NITRATE 250
- 5.11 AMMONIUM AND AMMONIA 252
- 5.12 CHLORIDE 254
- 5.13 TURBIDITY 255
- Optical Fiber 256
- Photodetector 256
- Light Source 256
- Near Infrared LED 256
- 5.14 CHLOROPHYLL 259
- IN VIVO 260
- IN VIVO CHLOROPHYLL 264
- 5.15 RHODAMINE WT 266
- Green LED 267
- Optical Filter 267
- PREPARATION 268
- (Orange LED) 270
- CALIBRATION METHODS 271
- SENSOR DRIFT 271
- GREEN ALGAE 275
- (Green LED) 276
- S•R•A•K=Q 281
- H•K+H•K=Q 282
- 6.1 CALIBRATION ERRORS 283
- Troubleshooting Section 6 284
- SECTION 7 COMMUNICATION 287
- 7.1 OVERVIEW 287
- 7.2 HARDWARE INTERFACE 287
- 7.3 RS-232 INTERFACE 288
- 7.4 SDI-12 INTERFACE 288
- Communications Section 7 290
- INFORMATION 293
- 9.1 LIMITATION OF WARRANTY 293
- 9.3 CLEANING INSTRUCTIONS 294
- 9.4 PACKING INSTRUCTIONS 295
- 9.5 PRODUCT RETURN FORM 296
- YSI Zobell Solution: 3682 300
- APPENDIX C ACCESSORIES AND 306
- CALIBRATION STANDARDS 306
- APPENDIX D SOLUBILITY AND 310
- PRESSURE/ALTITUDE TABLES 310
- APPENDIX F FLOW 320
- Flow Appendix F 321
- MANNING EQUATION 321
- DEPLOYING YOUR SONDE 322
- INSTALLING THE CARTRIDGE KIT 324
- INSTALLING THE CANISTER KIT 325
- APPENDIX H EMC PERFORMANCE 326
- EMC Performance Appendix H 327
- The “DOsat %” Convention 338
- APPENDIX K PAR SENSOR 342
- PAR Sensor Appendix K 343
- APPENDIX O SPECIFICATIONS 364
- 6600V2-4 SONDE 365
- 6920V2-1 SONDE 366
- 6920V2-2 SONDE 367
- 6820V2-1 SONDE 367
- 6820V2-2 SONDE 368
- 600XLM SONDE 369
- 600XL SONDE 369
- 600R SONDE 370
- 600QS SONDE 370
- 600 OMS V2-1 SONDE 371
- 600LS SONDE 372
- SENSOR SPECIFICATIONS 372
- Non-Vented Level – Deep 372
- in YSI AMCO-AEPA standards 374
- SOFTWARE SPECIFICATIONS 376
- Website: www.ysi.com 377
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